Investigation of Irisin (Fndc5) Gene Polymorphisms in Patients with Gestational Diabetes Mellitus

Caska H, Akbas H and Sak S

Published on: 2023-02-01

Abstract

Objective: Gestational Diabetes Mellitus (GDM) is defined as a disease of glucose intolerance, which is first identified in pregnancy. Transmembrane protein fibronectin type III domain-containing protein 5 (FNDC5) that is encoded by the FNDC5 gene has been demonstrated to be cleaved and released as a novel hormone-like myokine irisin. Irisin peptide, released from muscle cells, acting as a messenger between muscle and adipose tissue, has been known to play an important role in insulin resistance and energy metabolism regulation.

In this study, it was aimed to investigate the effect of two polymorphisms (rs726344and rs16835198) in the Irisin (FNDC5) gene, which is known to have an effect on serum irisin level, and their relationship with Gestational Diabetes Mellitus (GDM).

Methods: The study included 233 pregnanat subjects who applied to Harran University Medical Faculty Obstetrics and Gynecology Outpatient Clinic. The study population was divided into two groups; the first one is 110 subjects of the GDM group and the second one is 123 subjects of the control (Non-GDM subjects) group. 110 subjects of the first group, who diagnosed with GDM at the 24-28 gestational period, were chosen as the patient group according to the criteria of ADA (American Diabetes Association). DNA isolation was performed by using Genomic DNA isolation kit with spin column method from peripheral blood samples. Analysis of polymorphisms (rs16835198 and rs726344) of the FNDC5 gene of each subject was performed by using the Real-Time PCR. Two pairs of primers (forward and reverse) and two pairs of probes selected on the FNDC5 gene were synthesized according to sequence order and used to replicate genomic DNA in Real-Time PCR and the polymorphisms were genotyped by amplification curves gained from the RT-PCR results.

Results: As a result of the analyzes made, statistically no significant difference was observed in the genotype distribution and allele frequency of FNDC5 gene G>A (rs726344) and rs16835198 G> T polymorphisms in GDM patient and control groups (p>0.05).

Conclusions: As a result of this research, it was concluded that FNDC5 gene rs726344 and rs16835198 polymorphisms are not related to gestational diabetes mellitus.