Comparison of Cvemp in Dance Practitioners & Non Dance Practitioners
Jose S, Nithin Ak and Johnson SS
Published on: 2022-10-21
Abstract
Vestibular evoked myogenic potential (VEMP or VsEP) is a neurophysiological assessment technique used to determine the function of otolithic organs (utricle and saccule) of the inner ear. It complements the information provided by caloric testing and other forms of inner ear (vestibular apparatus) testing. There are two types of VEMPs. One is the oVEMP and the other is cVEMP. The oVEMP measures the integrity of the utricule and superior vestibular nerve, and cVemp measures the saccule and the inferior vestibular nerve (Manzari, Burgess & Curthoys, 2010).
Purpose
The current study was conducted with the aim of investigating the plasticity of the sacculocollic pathway in skilled dancers and non-dancers.
Method
Two teams of subjects,a management cluster and an experimental cluster, participated in the study. The experimental cluster was further divided into 2 subgroups- skilled dancers who have received coaching in Bharatanatyam dance; and skilled dancers who have received coaching in Salsa dance. The experimental cluster consisted of total Forty subjects (80 ears), and 20 subjects (40 ears) in every subgroup, management cluster consisted of thirty people (60 ears).
Results
The results revealed an enhancement in the amplitudes of P13, N23 and P13-N23, in skilled dancers compared with the normal management cluster. Results, but did not reveal any significant differencesin the latency of P13 and N23 peaks within the two teams. The difference in amplitude between the two teams was attributed to the sacculocollic pathway in the dancers.
Conclusion
The results showed a significant difference in terms of the amplitude of the VEMP responses between dancers and non-dancers. From the current study, it can also be concluded that dance practice may improve the sacculocollic pathway. The amplitude of the VEMP parameters may be a better tool for reviewing the physical properties of the sacculocollic pathway than the latency parameters.
Keywords
Vestibular Evoked Myogenic Potentials; Latencies; Amplitude; PlasticityIntroduction
Dance, specifically classical and Western dance, involves exercises that manufacture economical muscle power and motor coordination. Integration is required between the osteomuscular and central nervous systems (SNC), with kinaesthetic feedback acting as an associated adjustment part [1]. A dance promotes Head and trunk movements and displacement from the middle of gravity in all directions, and from the support axis allows the event of things that contribute to maintaining balance, such as joint quality and coordination [2]. Throughout dance, the necessity for constant positioning of the top in numerous directions in relevant gravity activates the vestibulospinal reflex, which causes offsetting displacement of the external body part phase in the direction opposite to body rotation, keeping the top stable [3]. VEMP could be a biphasic repressing response induced by loud clicks or tone bursts recorded from the tonically contractile musculussternocleidomastoideus, being the sole resource offered to assess the operation of the sac and inferior proprioceptive nerve [3-8]. The sensory pathway of the VEMP, which is mediated by the sacculus (the receptor), is responsible for linear acceleration associated with swiftness, and consists of an initial positive peak (P13, or p1) within the ipsilateral SCM followed by consecutive negative and positive peaks (N23, P34, and N44).
Method
Ethical approval was obtained from the Institutional ethical committee before the start of the study also an informed consent was signed from the participant explaining all the procedures concerned during this study. Participants: Two teams of participants participated in the study; an experimental cluster and a control cluster. The experimental cluster consisted of 40 skilled dancers (80 cars) within the age range of 16 to 40 years (mean age 29years) participated within the study. The experimental clusters were divided into two groups.
- Skilled dancers who have received coaching in Bharatanatyam [Total twenty subjects, (14 females, and 6 males)]
- Skilled dancers who received coaching in salsa [Total twenty subjects, 16 females, 4 males). On those dancers who had received coaching and performing continued with the dance application were included in the study. The management cluster, on the contrary, hand had 30 people (60 ears), with 18 females and a dozen males within the age range of 16 to 40 years (mean age of 24years). They did not receive any skilled coaching within the dance. Participant choice criteria for the experimental group: All participants had a minimum of one year of dance experience/regular application ofperforming arts. Thestandard for one year of dance application ispredicated relies} on studies that have shown that dance -based coaching improves balance in young adult subjects in three months [9]. The participants’ hearing sensitivity was within traditional limits (i.e., puretone average of 500 cycles per second, 1kHz and 2 kHz ≤15 dBHL). None of the participants had history or presence of any otological issues such as ear discharge, ear pain, itching, or tinnitus. In addition, they failed to have a history or presence of any neuromuscular downside, history, or intake of medication which would result in vestibulotoxicity, and had no symptoms associated with proprioceptive disorders. Participant choice criteria for the management cluster: Participants within the management group failed to have any formal dance experience or regular application of diversion. The participants’ hearing sensitivity was within traditional limits (i.e. the pure tone average of 500 cycles per second, one kHz and two kilohertz was ≤ 15dBHL). None of the participants had a history or presence of any otological issues such as ear discharge, car pain, itching, or symptoms; additionally, the participants failed to have any history of fasciculus downside history of intake of medication which will result in vestibulotoxicity, and had no symptoms associated with proprioception issues. To evaluate hearing sensitivity, tone audiometry was performed usinga MAICO MA42 twin channel measuring device. The air conductivity thresholds and bone-conduction thresholds were measured. The thresholds (minimum level of hearing) for air conductivity were measured usingTDH39 headphone (770 Park Ave, Huntington, NY11743, US)) and a B-71 bone vibrator (Radio ear, KIMMETRICS 22050 Mohawk Drive, Smithsburg, MD 21783) were accustomedto verify bone conductivity thresholds. The middle-ear operation was analysedusimg a GSI Tympstar instrument (GSI VIASYS aid, Wisconsin. USA). Vestibular induced myogenic potentials were recorded using Intelligent Hearing Systems (Smart EP) System (Intelligent Hearing System, Florida, USA) with an Insert ER-3A headphone (Etymotic Research, Inc. 61 Martin Lane, deer Grove Village, IL 60007 USA).
Procedure
A detailed history was obtained concerning the condition of the hearing system from all the participants. Tone thresholds were obtained by victimisation modified Hughson Westlake procedure [10], for octave frequencies from 250 Hz to eight kilohertz for air conductivity stimuli and from 250 Hertz to four kilohertz for bone physical phenomenon stimuli. For Tympanometry 226 Hertz probe tone was used, ipsilateral and contralateral reflexes at five hundred Hertz, 1000 Hz, 2000 Hertz and 4000 Hertz were obtained for each the ears. UCL for all the participants was obtained by presenting speech stimuli at one hundred dB, Participants were tutored to reply to the stimuli by voice communication whether or not it's comfy or not. Corrected VEMPs were recorded for each the teams by averaging of the sonically induced EMG of the musculus sternocleidomastoideus. Subjects were tutored to show their neck towards the non-stimulation ear facet i.e., to rotate towards the contralateral facet of the testing ear. a visible feedback was given to the participants so as to observe their musculus sternocleidomastoideus tension. The muscle tension was monitored with electromyogram level feedback system provided by IHS system. The electromyogram level was maintained between 100 to 2 hundred% (50µV to 100µV) for all the participants. The location of the conductor placement was ready with skin preparation gel, chloride disc electrodes with conducting gel was used. Absolute conductor impedanceof Five kilo-ohm and inter conductor impedances of fewer than two was maintained. Subjects were created to take a seat in upright position and were tutored to tense the musculus sternocleidomastoideus throughout runs of acoustic stimulation and relax between runs. The VEMP was recorded with the subsequent protocol: five hundred cycles/second tone burst stimulant was chosen supported the sooner studies that show a much better amplitude and response rate with 500Hz tone burst [11].
Response Analysis
VEMP was recorded for each ears for all the subjects. The responses were morphologically analysed to interpret the VEMP findings. 2 recordings were obtained for identical ear to confirm reliableness of the wave form. 1st positive peak and also the first negative peak of the biphasic wave with the latency of 13ms and 23ms was thought-about as p13 and n23 respectively, peak to peak amplitude was calculated so as to get amplitude of p13-n23 complex.
Table1: VEMP recording protocol.
|
Stimulus parameters |
Acquisition parameters |
|||||
|
Transducers |
Etymotic Research-3A |
Gain |
5000 |
|||
|
Stimulus type |
500Hz TB |
Time window |
-10 to70 ms |
|||
|
Intensity level |
95dBnHL |
filter setting |
30-1500Hz |
|||
|
Stimulus Polarity |
Rarefaction |
Electrode placement : |
||||
|
Number of sweeps |
200 |
Active |
Top half of sternocleido mastoid muscle |
|||
|
Rate of Repetition |
5.1/sec |
Reference |
Sterno-clavicular joint |
|||
|
Band reject filter |
off |
common |
forehead |
|||
Results
The latency and amplitude of various peaks of VEMP were analysed for the 2 teams i.e. the experimental cluster and the management cluster. Latency and amplitude of P13, N23 peak, amplitude of P13, N23 and peak to peak amplitude of P13-N23 complex were compared across the 2 teams. VEMP can be recorded for all the subjects in each the management and the experimental teams. P13 and N23 peaks within the wave shape were visualised and analysed for the management (non-dancers) cluster and therefore the experimental (dancers) cluster. To understand the cluster variations between the experimental and the management cluster, the info of 2 subgroups of the experimental cluster (salsa & bharatanatyam dancer group) was combined. The mean and variance (SD) were then computed for the management cluster and therefore the combined knowledge of the experimental cluster. The mean values for the latency of P13 and N23 for each the management and therefore the combined knowledge of the experimental cluster are given within the Table two.
Table 2: Mean and SD values of latency (msec) of control & the experimental group.
|
Parameters |
P13 latency |
N23 latency |
||
|
Groups |
Mean (msec) |
SD(msec) |
Mean (msec) |
SD(msec) |
|
Control |
15.88 |
2.38 |
21.42 |
2.29 |
|
Experiment |
15.46 |
1.79 |
21.22 |
1.62 |
As we are able to see from Table 2 that the mean latency for the
management cluster and also the combined information of the experimental cluster for the P13 peak and N23 peak are virtually similar. But it will be noted from Table 2 that the standard deviation within the experimental cluster is lesser than the management cluster. To understand the many variations within the mean values for the latency of P13 & N23 peaks, for the combined knowledge of the experimental and also the management cluster, independent sample‘t’ test was done. Independent sample t-test didn't show any important variations for the latency of P13. [t (68) =0.835, p>0.05) and N23, [t (68) =0.516, p>0.05] between dancers and also the non-dancers cluster. The experimental cluster had 2 subgroups (Salsa & Bharatanatyam group) one in all the subgroup concerned Indian sort of dance type and also the alternative subgroup concerned western sort of dance type. To know the variations between latency of the management cluster and also the 2 subgroups of the experimental cluster severally, mean and S.D were calculated for every cluster severally. The info about measure is given in Table three.
Table 3: Mean & SD of P13 & N23 latencies of vemp responses in control group and experimental group.
|
|
P13 latency |
N23 latency |
||
|
Groups |
Mean (msec) |
S D |
Mean (msec) |
S D |
|
Non dancers |
15.88 |
2.38 |
21.42 |
2.24 |
|
Kathak dancers |
15.36 |
1.48 |
20.55 |
1.28 |
|
Salsa dancers |
15.56 |
2.1 |
21.51 |
1.71 |
As it will be seen from the Table three, mean latency for P13 and N23 peaks are virtually similar for the management cluster and also the 2 subgroups of the experimental cluster It also can be seen from table that the standard deviation is once more less for the latency of each P13 furthermore as N23 peaks for the 2 subgroups of the experimental cluster. To any understand the variations between the management and also the experimental subgroups teams that is, the non-dancers, dancer bharatnatyam cluster and dancer salsa cluster, multiple analyses of variance (MANOVA) was done. Multiple analyses of variance failed to show vital main impact of cluster on latency of P13 [F(2, 67)=0.39, p>0.05) and latency of N23 [F (2, 67) =2.34, p>0.05].
Amplitude of P13, N23, and P13-N23 Complex
Amplitude of P13, N23 peak and peak to peak amplitude of P13-N23 complex within the wave form analysed for the non-dancers cluster and also the 2 dancers cluster. First the info of the 2 subgroups of the experimental cluster (i.e. Salsa & Bharatanatyam dance groups) was combined. This was done in order to know the general distinction between the amplitude of the P13 peak, N23 peak and also the P13-N23 complex for the management and also the experimental cluster. Mean and variance for amplitude of P13, N23 peak and peak to peak amplitude of P13-N23 complicated of VEMP responses for experimental and management teams were computed and also the details area unit given in Table four.
Table 4: Mean and SD of amplitude of P13 and N23 & peak to peak amplitude of P13-N23 complex of dancers and non-dancers group.
|
|
P13 amplitude |
N23 amplitude |
P13-N23 amplitude |
|||
|
Groups |
Mean (µv) |
SD |
Mean (µv) |
SD |
Mean (µv) |
SD |
|
Control |
16.06 |
8.42 |
21.48 |
10.12 |
37.49 |
17.38 |
|
Experimental |
23.55 |
6.22 |
27.44 |
9.21 |
50.98 |
13.23 |
It may be seen from Table four that the amplitude of the VEMP responses within the experimental cluster for P13, N23 and additionally for the P13-N23 complex is way higher compared to the management cluster. In addition it may also be seen that the standard deviation for the experimental cluster is once more low compared management cluster.To find out the many variations within the mean values for amplitude of P13, N23 and peak to peak amplitude of P13-N23 complex for the management and therefore the experimental cluster, independent sample t check was done. Independent sample t test showed vital variations for the amplitude of P13 peak [t(168)=4.309, p<0.05), N23 peak, [t(68)=2.572, p<0.05) and peak to peak amplitude of P13-N23 complex [t (68)=3.694, p<0.05) between the control and the experimental groups. To understand the variations for the amplitude of the management cluster and also the 2 subgroups of the experimental cluster severally, mean and S.D were calculated for every cluster singly. Mean and standard deviation values for amplitude of P13, N23 peak and peak to peak amplitude of P13-N23 complex for the management cluster (non-dancers) and also the 2 subgroups of the experimental cluster (Bharatanatyam and also the Salsa group) are given within the Table five.
Table 5: Mean and SD of P13 and N23 latencies in non-dancers and dancers.
>
|
|
P13 amplitude |
N23 amplitude |
P13-N23 amplitude |
|||
|
Groups |
Mean (µv) |
SD |
Mean (µv) |
SD |
Mean (µv) |
SD |
|
Control(non-dancers) |
16.06 |
8.42 |
21.48 |
10.12 |
37.49 |
17.38 |
|
Experimental (Bharatanatyam) |
25.74 |
5.16 |
28.4 |
11.4 |
54.1 |
14.98 |
|
Salsa dancers |
21.34 |
6.48 |
26.8 |
6.54 |
48.12 |
10.73 |
Itmay be seen from Table-5 that mean amplitude value for amplitude of P13, N23 and peak to peak amplitude of P13-N23 complex are higher for the 2 subgroups of the experimental cluster (Bharatanatyam & Salsa dance cluster) compared to the management group. It may be seen from table that the mean amplitude worth of P13, N23 and P13-N23 advanced is higher for Bharatanatyam dancers compared to the salsa dancers and non-dancers. Additionally to perceive the variations between teams i.e., the management cluster and also the 2 subgroups of the experimental cluster (Bharatanatyam dancer cluster and Salsa dancer group), multiple analysis of variance was done. MANOVA showed vital effect of cluster on the amplitude ofP13 peak [F(2, 67)=11.63, p<0.05], amplitude of N23 [F(2, 67)=3.41, p<0.05] and also amplitude of P13-N23 complex [F(2, 67)=7.69, p<0.05].
Discussion
To summarise the results, there was no important distinction in terms of P13 and N23 latency of VEMP response in each the management and also the experimental cluster. However, there was important distinction within the amplitude of P13, N23 peaks & peak to peak amplitude of P13-N23 complex of VEMP response in each non dancer cluster and dancer cluster that is; amplitude was considerably higher within the experimental cluster compared to the management cluster. The mean values for the latency of P13 and N23 peaks are in agreement with the previous studies [10]. However, there are studies that have reported completely different latency values for each P13 and N23 peaks [11-15] have reported the same latency as obtained within the present study. The protocol used and also the instrumentation employed in this study is same as that of employed by [16] and [17], whereas alternative studies have used {a completely different|aspecial|aunique|a distinct} protocol and different instrument to record the VEMP. The distinction within the latency of VEMP from the study by Wang and [18], [19] may be because of the actual fact that the instrumentations used and also the standardization variations in recording is also completely different from this study. There was no distinction within the latency of P13 peaks or N23 peaks among the management versus experimental teams. Numerous studies have reported that latency parameter of VEMP is comparatively less subject to bear changes than amplitude and threshold of VEMP response [20,21]demonstrated that there was no impact of any of the stimulation parameters (ie, stimulation level, stimulation frequency, and tonic myogram level) on latency of P13 and N23 of VEMP response. Other studies that concerned the study of degeneration method of the sacculocollic pathways have additionally reported no important modification within the latency parameters compared to the amplitude parameters [22-25]; [26]. Even the information that represents the various pathological conditions have reported no modification in latency [27-30]. Thus, the no modification in latency between the management cluster and experimental cluster may well be because of the very fact that the latency of the VEMP might not show a big modification within the latency parameters. There were statically important variations within the amplitude of P13, N23 and peak in peak amplitude of P13-N23 complex of VEMP responses between experimental and management groups. Enhanced P13, N23 amplitude and peak to peak amplitude of P13-N23 complex of VEMP responses were seen in experimental cluster than management cluster. The amplitude obtained for the management cluster within the present study is sort of like amplitude values reported in earlier studies [31,32]. Most of the studies reported that amplitude parameter of VEMP is comparatively more subject to bear changes than the latency of VEMP response. Pathological studies have additionally reported that the amplitude of VEMP is reduced or abnormally high (i.e, amplitude is additional susceptible to bear changes than latency) like disease [33], acoustic neuromas [34-40]. several behavioural studies reported that dance primarily based coaching improves that balance in young and adult subjects inside 3 months [41]. Thus, the variations in amplitude of the P13, N23 and P13-N23 may well be as a result of that amplitude changes within the experimental cluster would have been additional compared to the latency of the VEMP. The results obtained here indicate potential sacculocollic pathway plasticity thanks to the regular practice of the dance. The numerous improvements during amplitude of the VEMP responses might be because of the actual fact that the dance needs a lot of balance activity and so would have resulted in a lot of responsive sensory system within the dancers compared to the non-dancers. The plasticity within the sensory system would have occurred in numerous bodily structures which might be measured with the opposite techniques like Electronystagmography/ Videonystagmography. Here within the present study, solely otolith organs were assessed. Just like the physical property in different proprioception structures, the plasticity of the otolith organs would have occurred in dancers. Thus, the otolith organs would became a lot of responsive and an improved functioning of those structures would have resulted in improved amplitude responses of the proprioception induced myogenic potentials. Another factor to be noted within the present study is that the people who had got coaching within the Indian sort of dance type that's Bharatanatyam dancer had larger amplitude values compared to the western sort of dance i.e. salsa dance pattern. Classical ballet, thought of a physical activity that needs musculoskeletal acquisition, due to the advanced high-impact movements and huge joint vary of motion involved, additionally to developing coordination, balance and laterality associated with strength and suppleness of technical execution. Thus, developing these skills, related to the movements inherent to ballet, might end in improved vestibulospinal reflex development.
Conclusions
In the present study, the results discovered important distinction findings in terms of amplitude of the VEMP responses between the dancers and also the non-dancers. From the current study it is complete that the dance practice might improve the sacculocollic pathway. Amplitude of the VEMP parameters may be a higher tool to review the physical property of sacculocollic pathway compared to the latency parameters.
Acknowledgements
I would like to thank all the participants for participating in this study and sharing their information and experiences.
Data access statement
The data that support the findings of this study are available upon request from the corresponding author; [N.A.K]. The data are not publicly available due to [restrictions suchas containing information that could compromise the privacy of research participants.
Ethical Considerations
Ethical approval was obtained from the Institutional Ethical Committee before conducting the study, and informed consent was obtained from the participant explaining all the procedures involved in this study.
Funding
This research did not receive any grant from funding agencies in the public, commercial, or non-profit sectors.
Authors' contributions
N.A. K.: Study design, data collection, analysis and interpretation of results, and drafting of the manuscript.
S.J.: Data collection, analysis, and interpretation of results.
- S. J.: Data collection, analysis, and interpretation of results.
Conflict of interest
The authors do not have any financial or other interests related to this study.
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