Changes in Antioxidants Activities in Sarotherodon melanotheron Exposed to Butachlor under Laboratory Conditions

Orokotan OO, Akinrotimi OA, Ayaobu-Cookey IK, Ogbuzuru-Ebe LS and Omodu FA

Published on: 2025-06-07

Abstract

Butaforce is one of the most widely used butachlor-based herbicides in Nigeria for managing a range of weeds in agricultural operations. To evaluate the herbicide's oxidative effect, a biomarker test was conducted on Sarotherodon melanotheron plasma using some of the antioxidants. Some specific antioxidants, including glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), lipid peroxidase (LPO), and glutathione (GSH), were measured in the plasma of S.melanotheron exposed to the chemical in order to evaluate oxidative stress in fish exposed to different butachlor concentrations: 0.00 mg/l-control 0.05, 0.10, 0.15, and 0.20 mg/l. Using Randox test kits, blood samples from adult and juvenile S.melanotheron were used for the study. The results of the antioxidant study showed that SOD and GSH values significantly reduced (P<0.05), while CAT and LPO significantly increased in both sizes when compared to the control. Depending on the chemical's concentration, the juvenile fish displayed more pronounced alterations than the adult fish. The results are in line with assessing the risk of pollution to aquatic ecosystems by utilizing oxidative stress measurements in a comprehensive way.