Effects of Human Umbilical Cord Matrix Stem Cell Conditioned Media on Neural Cells Apoptosis in Cuprizone Mice Model of Multiple Sclerosis
Marzban M
Published on: 2023-12-12
Abstract
Background: Human Umbilical cord matrix stem cell (h-UCMSC) elicits neuroprotective effects, and their repair ability has been investigated in different experimental models. We aimed to investigate the effect of h-UCMSC supernatant or conditioned media in the cuprizone model of multiple sclerosis in mice.
Method: Adult male C57BL/6 mice (n = 40) were fed a regular diet or a diet containing cuprizone (0.2% w/w) for six weeks. h-UCMSC was cultured and supernatant was collected. h-UCMSC supernatant was administered intraperitoneally for two consecutive weeks at the end of the fourth week of cuprizone administration. Animals (n = 12) were perfused with normal saline at the end of sixth week. We used tunnel staining in corpus callosum to count apoptotic cells. Result: h-UCMSC conditioned media decrease apoptotic cells in corpus callosum, Conclusion: Our data indicated a remyelination potency of multiple i.p. hUCMSC supernatant in the cuprizone model of multiple sclerosis in mice.