Oxidative Stress in Tilapia guineensis Exposed to Glyphosate in the Laboratory

Okenwa U, Eze BU, Nwosu P and Ogolo C

Published on: 2024-07-26

Abstract

Roundup is among the most widely used glyphosate herbicides in Nigeria for a range of weed control in agricultural operations. A biomarker test was conducted on Sarotherodon melanotheron plasma to evaluate the oxidative impact of the herbicide using a portion of the antioxidants. Specific antioxidants, including glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), lipid peroxidase (LPO), and Glutathione (GSH), were measured in the plasma of S.melanotheron exposed to Roundup in order to evaluate oxidative stress in fish exposed to different concentrations of the chemical: 0.05, 0.10, 0.15, 0.20, and 0.25 mg/l. Both juvenile and adult Sarotherodon melanotheron had their blood samples drawn, and Randox test kits were utilized for the study. The results of the antioxidant analysis showed that SOD and GSH values dropped significantly (P<0.05), although CAT and LPO were significantly increased in both sizes when compared to the control. Compared to the adult fish, the juvenile fish displayed more pronounced alterations, and these changes were concentration-dependent. The results obtained align with the integrated application of oxidative stress parameters in the assessment of pollution risk to aquatic ecosystems.