Molecular and Biochemical Insights into Morus nigra Extract: Its Role in Antioxidant Defense and Vascular Protection

Abdullaev A, Gayibov U, Gayibova S, Abduazimova D, Omonturdiev S and Aripov T

Published on: 2025-10-31

Abstract

Oxidative stress is a key factor contributing to vascular dysfunction and metabolic disturbances. Morus nigra extract, rich in bioactive compounds, is recognized for its antioxidant potential, yet its precise effects on vascular tone and oxidative stress parameters remain underexplored. This study evaluates the impact of Morus nigra extract on oxidative stress markers, vascular function, and mitochondrial stability using an alloxan-induced model, highlighting its potential as a protective agent. The investigation included assessments of aortic contraction, creatine kinase activity, malondialdehyde (MDA) levels, antioxidant enzyme function, mitochondrial swelling, acute toxicity, and secondary metabolite profiling. The experiments were conducted on an oxidative stress model induced by alloxan. Morus nigra extract exhibited strong antioxidant properties, reducing MDA accumulation and enhancing the activity of key antioxidant enzymes. Additionally, the extract influenced vascular responses by modulating aortic contractility and protected mitochondrial integrity under oxidative stress conditions. Acute toxicity analysis confirmed its safety profile, while phytochemical screening identified secondary metabolites that may contribute to its bioactivity.The findings support the potential of Morus nigra extract in mitigating oxidative stress and preserving vascular function. Its antioxidant and vasoprotective effects warrant further investigation for potential therapeutic applications.