Eco-Friendly Facile Spectrophotometric Determination of 5-Fluorouracil in A Spiked Human Blood Plasma, Pharmaceutical Preparations, and Spiked Environmental Waste-Water Samples

Ahmad NR and Rahman GN

Published on: 2025-09-24

Abstract

A simple, accurate, precise, rapid, economical, and sensitive ultra violet spectrophotometric method has been developed for the determination of 5-Fluorouracil in pharmaceutical preparations and environmental wastewater samples, which shows maximum absorbance at 268 nm in distilled water. Beer's law was obeyed in the range of 1-20 μg/ml, with a molar absorptivity of 1.35x10⁴ L.mol^-1.cm^-1, the relative standard deviation of the method was less than 1.5%, and the accuracy (average recovery %) was 100 ± 1.2. The Sandal’s sensitivity of the colored complex is 18.346 ng/cm², and LOD and LOQ are 2.725 and 8.992 ng/cm², respectively. No interference was observed from common excipients and additives that often accompany 5-Fluorouracil in pharmaceutical preparations. The method was successfully applied to the determination of 5-Fluorouracil in pharmaceutical formulations (creams), biological blood samples, and industrial wastewater samples. The proposed method was validated by sensitivity and precision, which proves suitability for the routine analysis of 5-Fluorouracil in true samples.