Development and Validation of a Dissolution Method for Lamotrigine Tablets by UPLC

Sangeetha S, Alexandar S, Kumar M and Jaykar B

Published on: 2019-02-28

Abstract

The aim of the present work was to develop an accurate, precise, reproducible and economical development and validation of a dissolution method for lamotrigine tablets by uplc. The uplc method was carried out using buffer and acetonitrile 75: 25% v/v. The flow rate 0.3 ml/min with UV detection at 310 nm. Column used was acquity UPLC BEH Shield RP18, 2.1 X 50 mm. Assay percentage was found to be 100.8.  The method was validated according to International Conference on Harmonization guidelines and successfully applied to marketed linearity was plotted from (100 µg/ml to 300 µg/ml) (r2 = 0.9979) Lamotrigene formulations. The method was found to be linear in the respectively. Satisfactory values of Percent relative standard deviation for the intra-day and inter-day precision studies indicated that method is precise. Literature review revealed enormous analytical methods were reported for the estimation of lamotrigene but there is no development and validation of a dissolution method for lamotrigine tablets by uplc.