Spectrophotometric Assay of Tetracycline Hydrochloride in Pharmaceutical Preparations and Spiked Industrial Waste-Water Samples. Application to Content Uniformity Testing

Naif Rahman G and Rahman Ahmad N

Published on: 2024-12-28

Abstract

A simple, accurate, and rapid visible spectrophotometric method has been developed for the determination of tetracycline hydrochloride in pure, pharmaceutical preparations and environmental water samples .The method is based on the direct determination the absorption maximum at 360 nm in distilled water.  Beer’s Law was obeyed in the range of 2-3mg/ml with molar absorptivity of 1.6113×10⁴ L.mol.^-1.cm^-1.  The relative standard deviation of the method was less than 2% and accuracy (average recovery ) was  100 ± 0.85 % .The optimum conditions for all colour development are described and the proposed method has been successfully applied for the determination of  tetracycline hydrochloride in pharmaceutical preparations and waste water samples. Application to Content Uniformity Testing.