The First Report on Polyhydroxybutyrate, a Natural Biopolymer from Pseudomonas nitritireducens, an Endophytic Bacteria Isolated from Grey Mangrove of Gulf of Kachchh, Gujarat, Western India

Jayanthi G, Karthikeyan K, Monika RS and Vijay Kumar V

Published on: 2024-12-24

Abstract

Polyhydroxybutyrates (PHBs) are biodegradable polyesters synthesized by many endophytic microbes and play a predominant role as bioplastic due to their hydrolysable ester bonds. The present study explores the advantages of PHB producing endophytic microorganism isolated from the grey mangrove Avicennia marina from coastal area of Mandvi, Kachchh. Sodium hypochlorite methods were employed to extract PHB from all isolates. The effective PHB producing endophytic bacterial strains were isolated and screened out by staining with Sudan Black B followed by N₂ estimation. Among 28 isolates, 10 showed positive result towards PHB. Among the isolates, maximum yield of PHB percentage was maximum in JJ07 strain with 72.37% in Nutrient Broth (NB) and 48.29% in Mineral Salt Broth (MSB) medium. Thin films of bioplastic were also developed. The potent isolates were subjected to molecular characterization by 16S rRNA sequences followed by BLAST and identified as Pseudomonas nitritireducens and submitted to the NCBI GenBank and received the accession number as MF351832. The chemical structure of the polymer synthesized by the potent strain P. nitritireducens was determined by UV, FT-IR and GC-MS analyses.