The Anti-Hrp Protein (Invertebrate Primitive Antibody) In Asterids and Ophuirids

Leclerc M

Published on: 2024-12-21

Abstract

The sea star anti-Hrp protein (Invertebrate Primitive Antibody: IPA) is produced aftrer immunizations of sea stars (Asterina gibbosa [1]. Asterias rubens) with the antigen HRP (Horse-Radish Peroxydase). Plasmocytes issued from sea star B cells [2] secrete a specific immune protein: an anti-HRP primitive antibody. This last one can be cloned from the sea star IGKappa gene in a recombinant manner .It is specific of the antigen HRP after Elisas [3]. The sea star IGKappa gene shows CDR1, CDR2 determining regions [4] and a complicity with CDR3 region as it can be observed in 3D modelizations [5]. We recall also the existence of Ophiocomina nigra(Ophuirid) IGKappa gene [6].

Keywords

Anti-Hrp Protein

Introduction

Figures 1, 2, 3: Sea Star Immune Cells in TEM: You Put the 3 Images... And So On I Bis) the Asterid of Mediterranee: "Echinaster Sepositus".

Figures 5, 6: Sequencing DNA and AA cids of the sea star IGKappa gene.

With the translation

gga tcc gga gga atg cgt ggc aac atg gcg tct cta tgg atg ttc ttc ttt gtc gtg ggg
G   S   G   G   M   R   G   N   M   A   S   L   W   M   F   F   F   V   V   G
ata act tta caa cgg agt ttg gcg att tac acg ttt cgc gag caa ccg tcg gac act agc
I   T   L   Q   R   S   L   A   I   Y   T   F   R   E   Q   P   S   D   T   S
gcg ttg cag ggg agc aca gtg gtg ctt cac tgc tcc gtt gag cag tac ata aac acc acg
A   L   Q   G   S   T   V   V   L   H   C   S   V   E   Q   Y   I   N   T   T
gcc atc gtt tgg tgg agc cgt gac tcg gtc atc agc cac aac aaa gac ctg aaa ctg tcc
A   I   V   W   W   S   R   D   S   V   I   S   H   N   K   D   L   K   L   S
agt cta aac acc gac cag ctc caa agg tac tcg att tca ggc gac gca tct cgg ggg gaa
S   L   N   T   D   Q   L   Q   R   Y   S   I   S   G   D   A   S   R   G   E
ttc aac ctt aaa ata gtg aac ttt acc gcc aca gac gcc gcc agt tac cgc tgt cag atg
F   N   L   K   I   V   N   F   T   A   T   D   A   A   S   Y   R   C   Q   M
taa gaa ttc

- E F.

Figures 7, 8: The CDR1, CDR2, CDR3 Regions of the IPA (Invertebrate Primitive Antibody) From IMGT Montpellier (France).

Results

Table 1,2 : Issued From IMGT Resume the Following Analysis Below: I).

>starfish|IGKV1-5*01|33.3|||Pongo abelii

.....EQPSDTSALQGSTVVLHCSVEQYI.....NTTAIVWWSRDSVISHNKDL.KL.......SSLNTDQL.QRYSISGDASRGEFNLKIVNFTATDAASYRCQ.......................

The conserved amino acids (positions 23, 41, 89, 104) are found in the starfish sequence.

This molecule appears to have an IG AA sequence as seen from the above analysis.

If it aligns with the Pongo IGKV1-5, the percentage of alignment is 33%, so it is a sequence that seems to have similarities to an IGKV gene when it comes to conserved amino acids.

It appears clearly that CDR1 and CDR2 exist in the sea star primitive antibody and less clearly for CDR3 (1 amino acid which is conserved)

AlphaFold prediction of IGKV1-5*03 Homo sapiens in black sea star CDR1, CDR2, CDR3 (at the left) determining regions

Figure 9: Modelization 3D of the IPA (Invertebrate Primitive Antibody).

Figures 10, 11: The Anti-HRP (Horse-Radish Peroxydase) Antibody In Ophiocomina Nigra (Ophuirid. Echinoderm) The Anti-HRP Is Colored In Brown At The Level Of " Positive" Plasmocytes.

Fig 11: DNA Sequence of the Ophuirid Igkappa Gene.

Journal of Medical and Clinical Studies

The Anti-Hrp Protein (Invertebrate Primitive Antibody) In Asterids and Ophuirids

Abstract

Keywords

Introduction

Results

References