A simple Eco-Friendly Novel Estimation of Folic Acid in pharmaceutical preparations and environmental wastewater samples: Application to Content Uniformity Testing

Rahman Ahmed N and Naif Rahman G

Published on: 2024-12-28

Abstract

A simple, accurate, precise, rapid, economical and sensitive spectrophotometric method has been developed for the determination of Folic acid in pharmaceutical preparations and environmental wastewater samples, which shows maximum absorbance at 283 nm in 0.1 N Sodium hydroxide. Beer's law was obeyed in the range of 0.2-1.6 mg/100 ml ,with  molar absorptivity of 2.258x105  L.mol-1.cm-1 , relative standard deviation of the method was less than 1.2%, and accuracy (average recovery %) was 100 ± 1.0  .The method was successfully applied to the determination of Folic acid in pharmaceutical formulations (tablets),application to content university testing , and industrial wastewater samples. The proposed method was validated by sensitivity, and precision which proves suitability for the routine analysis of Folic acid in true samples.