The Antibiofilm Activity of Ziziphus Spina-Christi and Nigella Sativa Aqueous Extract against Some Gram Positive and Gram Negative Bacteria

Al-Nuaimy WA

Published on: 2024-12-05

Abstract

Introduction: Plants could be a solution for the growing issue of antibiotic resistance and the lack of new antibacterials. Plants possess a variety of efficient defense mechanisms, including the synthesis of secondary metabolites, to counteract pests and pathogens before they can inflict significant harm.

Objective: This research was focused to study the antibiofilm activity of Ziziphus spina-christi and Nigella Sativa aqueous extract against some bacteria under aerobic and anaerobic condition.

Methods: the isolated that used in this study Staphylococcus aureus, Enterococcus feacalis, lactobacillus spp, Proteus mirabilis, Citrobacter freundii and Pseudomonas aeruginosa which obtained from the microbiology laboratory in Al Hassein teaching hospital, all of the aqueous extracts were prepared for Ziziphus spina-christi and Nigella sativa seeds then determination Minimum inhibitory concentrations (MIC) of plants extract, and identification the effect MIC of extracts on preformed biofilm under aerobic and anaerobic condition.

Results: the results showed the MIC of the extracts showed variations between isolates under aerobic and anaerobic condition in both extracts, Nigella sativa extract revealed anti biofilm activity against the studied isolates and there was no significant difference of Nigella sativa antibiolfim in anaerobic and aerobic except in P. aeruginosa, the antibiofilm was lower in aerobic condition (0.212+0.00) than anaerobic (0.280+0.026) as well as in the biofilm in P, mirabilis in aerobic condition (0.207+0.064) was lower than anaerobic (0.234+0.044). However the biofilm formation in E.feacalis in an aerobic (0.208+0.005c) was lower than anaerobic (00.253+0.016b). The results showed that the Ziziphus spina-christi extract revealed a good anti biofilm activity against the studied isolates also the results found there was no significant difference of Ziziphus spina-christi antibiolfim in anaerobic and aerobic except in P. aeruginosa, the biofilm formation in aerobic was (0.222+0.00) lower than anaerobic (0.290+0.026).

Conclusion: Nigella sativa and Ziziphus spina-christi extract revealed a good anti biofilm activity against pathogenic bacteria.